Kwetu Foundation over the years has worked with different partners on issues of HIV and AIDS. Our HIV and AIDS programs focus on youth friendly HIV prevention campaigns and messaging, HIV testing, ART referrals, and Young People Living with HIV (PLHIV). Kwetu Foundation. Has a youth friendly HIV testing and counselling center at the offices, where young people are free to walk into everyday to access information, HIV counselling, get condoms, PEP, and other HIV services.

Furthermore, the focus is more on interlinkages between HIV and other issues affecting women and girls e.g. gender-based violence, access to treatment and health services as well as health promotion. The organization also focus on emerging issues surrounding HIV that include TB/HIV co-infection and cervical cancer.